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These tests start with a shorter exposure period (4 hr or less) to the material of concern and then evaluate simply whether tissue has been killed or not (or, in other words, if necrosis is present or not). It should be clear that, if a material is found to have less than severe dermal irritation potential, it will not be corrosive and, therefore, need not be tested separately for the corrosion endpoint. 02 in. 16 in. on the back, palms, and soles of the feet (Hipp, 1978). The epidermis, the outer portion of the skin, is several layers thick, covers the entire surface of the body, and is referred to as the horny layer or stratum corneum.

183-184. Liebert, New York. , and Krag, S. S. (1986). An in vitro screen for potential inflammatory agents using cultured fibroblasts. In Alternative Methods in Toxicology, (A. M. ), Vol. 3, pp. 74-85. Liebert, New York. Matoltsy, A. , Downes, A. , and Sweeney, T. M. (1968). Studies of the investigation of the chemical nature of the water barrier. J. Invest. Dermatol. 50, 19. Marzulli, F. , and Maibach, H. I. (1975). The rabbit as a model for evaluating the skin irritants: A comparison of results obtained on animals and man using repeated skin exposures.

Rabbit screening procedure 1. A group of at least 8-12 New Zealand White rabbits is screened for the study. 2. All rabbits selected for the study must be in good health; any rabbit exhibiting snuffles, hair loss, loose stools, or apparent weight loss is rejected and replaced. 3. One day (at least 18 hr) prior to application of the test substance, each rabbit is prepared by clipping the hair from the back and sides using a small animal clipper. A size No. 10 blade is used to remove the long hair and then a size No.

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