By Prof. Dr. Christian Rittner, Dr. rer. nat. Peter M. Schneider (auth.), Prof. Dr. Christian Rittner, Dr. rer. nat. Peter M. Schneider (eds.)

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Extra info for Advances in Forensic Haemogenetics: 14th Congress of the International Society for Forensic Haemogenetics (Internationale Gesellschaft for forensische Hämogenetik e.V.), Mainz, September 18–21, 1991

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Some of the problems in determining a match in fragment size have been eliminatcd by switching from RFLP analysis to the PCR, but there still exists the possibility of lane-to-lane differences in DNA mobility. In order to alleviate this problcm, we ha ve uscd a size standard, which can be coelectrophoresed in the same Iane as the sample. By this technique, the size of a sample is determined by the way it migrates in relation to the known sizes of the fragments of the standard that are subject to the same effects in the electrophoretic Iane as the sample.

3, indicated that the fetus of a victim of gang rape was not the progeny of the husband, and termination of the pregnancy was elected. In another case counseling a s to the powe r of the me thod resulted in the victim dropping the r a pe charge s a nd identifying a consensual partner. Forensic identification services have been provide to the Armed Forces Institute of Pathology by a Collaborative effort wi t h Cellmark Diagnostics . The circumstances o f aircraft crew submersion ( 1 month) , scud a nd weapon explosions and the retur n of air crew r emains allowed for the us e of PCR bas ed testing to help verify identifications.

Since we do not seek a specific chrornosorne localization of an STR and we are early in developrnent of rnarkers, a shotgun approach continues to be the rnost rapid. Sheared hurnan DNA is cloned into a DNA sequencing vector, plated at low density and those containing one of five repeats identified by hybridization of a radioactive oligorner of 30 bases in length. Using stringent conditions only those predicted to have more than eight repeats in tandern are detected. Sequencing the ends of the clone provides the flanking sequence to the STR, necessary for fashioning a pair of prirners for polyrnerase chain reaction (PCR) arnplification.

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